Research on fabricating a novel scaffold of photo-curable PGSA+PCL-DA for liver tissue engineering

• Main Authors: Rui-En Du, 杜睿恩
• Other Authors: Yih-Lin Cheng
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/34skq5

碩士 === 國立臺灣科技大學 === 機械工程系 === 105 === Currently, the treatment of liver disease need organ transplantation, but the risk of surgery and the shortage of liver supplement are still the drawbacks which can’t be overcome. Recently, liver cell transplantation has become an alternative way to liver transplantation, can be used to treat liver disease. However, previous study found that cells may easy to fall directly into the bottom when culturing in the porous scaffold so that cells are not easy to stack and grow. This study designs a new scaffold to compare with another scaffold which used in the past experiment and through the way of dyeing to enhance the accuracy of scaffold. Hope to improve cell viability and proliferation. The main materials are PCL-DA and PGSA. The effects of different crosslinking densities were compared by tensile tests, contact angle tests and thermal properties analysis. Add the Sudan Black to improve the accuracy. At the concentration of 0.04wt%, the accuracy error can be controlled at 1~2%. After the results of the above-mentioned test, PGSA60 + PCL-DA was used to fabricate scaffold and the hepatocytes culture. It was found that the step-type scaffold has better cell viability due to it has more area which the cells could attach.