Analysis of Inhibition Effects Using Momordicin I from Momordica charantin Extract on Human Lymphoma Raji and Daudi

• Main Authors: WU, CHUN-TING, 吳俊廷
• Other Authors: KAO, YU-LIN
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/84376811161425413781

碩士 === 國立高雄大學 === 生命科學系碩士班 === 105 === In the world, cancer is ranking as the first to cause dead. Recently, the numbers of death rise caused by lymphoma. In Asia, non-Hodgkin’s lymphoma disease is the most among all causes of lymphoma. The onset symptoms of lymphoma are not obvious, and it is usually diagnosed as the late 3rd or 4th stage. Because of rapid deterioration, poor prognosis and high mortality occurred frequently afterwise. Therefore, finding a drug to inhibit the hasty proliferation of lymphoma cells is urgent. Two non-Hodgkin's Burtkitt’s lymphoma cell lines, Raji and Daudi, were used as experimental materials in this study. Bitter gourd, Momordica charantia, is a vegetable rich with nutrition, and known as a traditional medicinal plant with bioactive ingredients. It was reported with anti-cancer function. The functions of purified Momordica charantin triterpene extract momordicin I had been tested in this research, including cell viable rate, proliferation, apoptosis and cell cycle regulation. The results showed that viable and proliferation rate of Raji cells was significantly decreased in the pattern of dose-dependent. When the concentration is higher than 10 μM, the proliferation rate decreased dramatically. After treatment of momordicin I on Raji, cells exhibited early and late apoptosis. In the analysis of cell cycle progression, fewer cells remain in the stage of G0/G1 and more arrest at S phase. In the Western blotting, p-Akt and Akt decreased, and so did p-p53 and p53, implied that DNA damage might not been repaired and cell could turn into apoptosis pathway. Momordicin I caused mitochondria membrane potential abnormal. Anti-apoptotic protein Bcl-xL decreased above 10 μM momordicin I, besides, apoptotic protein Bax increased, apoptotic marker cleaved-caspase-9 and cleaved-caspase-3 increased, both of evidences coincident the apoptosis of Raji cells is triggered by momordicin I. In the cell line of Daudi, the viable and proliferation rate were significantly decreased, and the IC50 concentration is 5.19 μM. These results showed that Daudi is more sensitive to Momordicin I than Raji. Early and late apoptosis significantly increased. The protein expression of p-Akt and Akt decreased, however, p-p53 and p53 increase. Anti-apoptotic protein Bcl-xL decreased, nevertheless, Bax and cleaved-caspase-3 increased, these results also suggest that apoptosis can be induced by momordicin I. The results of two lymphoma cell lines show that momordicin I possesses ability not only inhibiting proliferation, but also causing apoptosis. These results suggest that momordicin I has the potential to develop into a drug using in the treatment of lymphoma.