The antioxidant and whitening activities of Cistanchedeserticola Extract

• Main Authors: YANG,SHU-TING, 楊舒婷
• Other Authors: CHANG,CHUN-SHENG
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/15921893717820851378

碩士 === 南臺科技大學 === 生物科技系 === 105 === Cistanche deserticola is a worldwide genus of holoparasitic desert plant, and major distributed in Asian countries. The phenylethanoid glycosides (PhGs) are the major bioactive compounds of C. deserticola which showed the diverse pharmacological activities such as neuroprotective, anti-inflammatory, immunomodulatory, antioxidant, antibacterial and antitumor effects. In this study, we compared ultrasonic and general extraction with different concentrations of alcohol to extract PhGs and investigated the biological activities of PhGs including antioxidant and whitening capacity. The results showed that the highest concentration of PhGs that extract from dried C. deserticola with ethanol under mild condition was 29.8 mg PhGs/g cistanche deserticola extract. In the antioxidant capacity, the EC50 concentration of ABTS and DCFH-DA methods were 1.42 mg /mL and 6.39 mg /mL from the extract of C. deserticola, respectively. The IC50 concentration of inhibited melanoma cells and tyrosinase activity assay was 1.60 mg/mL and 11.47 mg /mL from the extract of C. deserticola, respectively. The 1.25 mg/mL extract of C. deserticola treated the melanoma cells can reduce 25% of the production of melanin during 72 hours. We confirmed that the extract of C. deserticola showed excellent whitening and antioxidant capacity.