The role of Prostate apoptosis response-4 (Par-4) in oral squamous cell carcinoma

• Main Authors: KUO, YEN-CHUN, 郭妍均
• Other Authors: LEE, JENG-WOEI
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/du92j2

碩士 === 慈濟大學 === 生命科學系碩士班 === 105 === Reduced expression level of prostate apoptosis response-4 (Par-4) gene has been demonstrated in multiple tumors. Intriguingly, restoration of Par-4 in tumor cells or loss of expression in transgenic animal model further indicates a tumor suppression activity of Par-4. However, until now, relationship between Par-4 and oral squamous cell carcinoma (OSCC) is still unknown. Here, via using immunohistochemistry and Western blot analysis, in contrast to lower expression and cytoplasmic distribution in normal cells and pre-cancerous lesions, higher intensity of Par-4 remarkably revealed a distinctive nuclear localization in tumor cells. Of interest, Par-4 induction elevated mitophagy mediated PINK1-Parkin dependent mitophagy pathway in OSCC cells was also manifested by TEM and immunofluorescence assays. In conclusion, our results suggested that Par-4 expression level and Par-4-mediated mitophagy could contribute to OSCC tumorigenesis.