| Summary: | 碩士 === 國立臺北科技大學 === 生化與生醫工程研究所 === 105 === Reactive oxygen species (ROS) are involved in many metabolic pathways. Hence, ROS is important for living cells. However, ROS which exceed the limit will be hazardous. In this study, we used DNA modified gold nanoparticles and Fenton reaction to develop a two-steps colorimetric detection for hydrogen peroxide (H2O2). In step 1, we used ferrous ion and hydrogen peroxide to produce hydroxyl radical (OH·) by Fenton reaction. In step 2, DNA modified gold nanoparticles were added and hydroxyl radical attacked DNA. It breaks phosphodiester bond on DNA and decreases the quantity of DNA on the surface of gold nanoparticles. This decrease caused gold nanoparticles aggregation. The color of solution changed from red to purple. In this study, the detection limit is 1μM and it takes 15 minutes for detection. We can directly determine the results by naked eye and without any instrument. This method is fast, easy operation and low-cost. To be a potential assay.
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