The potential role of Wedelolactone in treating particle-induced osteolysis

• Main Authors: Wen-Yu Chen, 陳玟聿
• Other Authors: 盧永昌
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/a64sr3

碩士 === 國立臺北科技大學 === 化學工程與生物科技系生化與生醫工程碩士班 === 105 === Ultra-high molecular weight (UHMWPE) wear particle-induced osteolysis has been recognized as the major problem of long-term failure in total joint replacement. These particles stimulated a serious immune reaction resulted in osteolysis lead to aseptic loosening. In clinical, many products using different material as component but still can’t avoid from generating wear particles. Therefore, The inhibition of osteoclast formation and function, and inhibitionof the secretion of inflammatory factors, are key targets for therapeutic agents in the treatment of wear particle-induced osteolysis. Wedelolactone (WDL), one of active ingredient of Chinese herbal medicine named Wedelia chinensis which is indicated having potential in treating osteoporosis. WDL has attracted considerable attention because of its important biological and pharmacological activities. The aim of the this study was to understanding of WDL as a therapeutic agent to prevent wear particle-induced osteolysis in vitro and in vivo. In this study, we applied micro-fabricated surface texture to rub against the UHMWPE pins to generate wear particles. In vitro study, we evaluated the effects of WDL on osteolysis by MTT test and ELISA test to analyze the biocompability and inflammatory cytokines. As a result, the WDL groups decrease the secret of inflammation factor. WDL may has the potential for inhibition of inflammation in vitro. In vivo study, the mice calvarial implement operation which injections of suspension of particles. After post-surgery 2 weeks. The mice were gavage feeding of 4 mg/kg/day WDL for 2 weeks. All calvarial were collected and analyzed by micro-CT, H&E stain and TRAP stain. In conclusion, these results demonstrate that WDL could be a potential treatment for prevention wear particle-induced osteolysis. However, its effect on osteoclasts and mechanism of action require further investigation.