Functional characterization of the polyketide synthase genes of Bacillus subtilis

• Main Authors: ZHUO, JING-WEI, 卓靖爲
• Other Authors: SHU, HUNG-YU
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/a296hf

碩士 === 長榮大學 === 醫學研究所 === 106 === Polyketides are synthesized by the polyketide synthases (PKSs) megacomplex. Many polyketides can inhibit the growth of fungi and bacteria. In addition to the PKS genes, sfp gene is also required for polyketide biosynthesis. Sfp converts apo-form PKSs into holo-from PKSs by posttranslational modification. This study was to investigate the function of the pksF gene cluster found in Bacillus subtilis F29-3. Mutagensis analysis shown that both pksF and sfp gene were required for a polyketide (Polyketide-F) synthesis. Observation by scanning electron microscopy revealed that the size of the pksF mutant was 60% of that of the wild strain. The sporulation of the wild-type strain and the pksF mutant strain was analyzed. The wild-type strain did not produce spores within 24 hours, and the pksF mutant strain had produced a large number of spores in 12 hours. B. subtilis F29-3 and mutant strains were co-cultured with Phaseolus vulgaris HV177 and Arabidopsis thaliana Col-0, and plants root length were longer when coculture B. subtilis F29-3. The results show that the pksF gene cluster is involved in Polyketide F synthesis and the genes of pksF affect the sporulation ability, root length in plants and the size of the bacteria.