| Summary: | 博士 === 中國醫藥大學 === 中醫學系博士班 === 106 === Epileptic seizure can induce inflammation in brain, and this inflammation can enhance neuronal excitation, thus, anti-inflammation became as a ploy for antiepileptic treatment. Three yang meridians of hand and foot connect with ear, and the 7th, 9th and 10th cranial nerve that have parasympathetic components also distribute to ear. Therefore, the purpose of the present study was to investigate: 1) the effect of long-term electric stimulation at ear and electroacupuncture (EA) at Zusanli-Shangjuxu (ST36-ST37) on inflammation; 2) the role of toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) signal pathway in 2 Hz and 15 Hz electric stimulation (ES) at ear. The present study established a kainic acid (KA)-induced epileptic seizure model, ES at ear and EA at ST36-ST37 for 6 weeks, and 2 Hz and 15 Hz ES at ear for 3 weeks. The results indicated: 1) 2 Hz ES at ear and EA at ST36-ST37 for 6 weeks can reduce COX-2 levels of hippocampal CA1, and also can reduce astrocyte and S100-B immunoreactivity cells; 2) 2 Hz and 15 Hz ES at ear for 3 weeks can reduce TLR4, pCaMKIIa??, pERK, pp38 and pJNK, and pNK-kB expression of frontal cortex, hippocampus and cerebral cortex.
Based on the mentioned-above results, long-term ES at ear or long-term EA at ST36-ST37 can reduce inflammatory reaction induced by KA-induced epileptic seizures. In addition, the results also indicted 2 Hz or 15 Hz at ear for 3 weeks can reduce inflammation in brain. Thus, suggesting different frequencies or long-term ES at ear or EA at ST-36-ST36 were benefit for epilepsy treatment.
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