Application of sakura extracts in sunscreen cosmetics

• Main Authors: Te-En Huang, 黃得恩
• Other Authors: Lin,Ching-Gong
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/797ckh

碩士 === 嘉南藥理大學 === 化粧品應用與管理系 === 106 === Summary Among the flowers of the East, the cherry blossoms are like the roses of the Western countries, full of many magical effects and legends! Unfortunately, there is not much literature on the efficacy of extracts of flowering cherry trees. Secondly, the comprehensive study on the efficacy of various parts of the cherry tree is very scarce! The purpose of this study was to investigate the antioxidant capacity of the flower, leaf, stem/coarse branch and twigs extracts of the flowering cherry tree (Sakura), the ability to remove melanin, the DNA protection activity. As well as the ability to regenerate cells, we have developed an environmentally friendly sunscreen that is safe, effective and friendly. The main cherry tree is commonly known as Taiwan Cherry Blossom. Different fractions of the extract were prepared by using RO water as a solvent while heating in a microwave oven. There were four extracts. The results showed that the antioxidant activity was determined by the decolorization method of 1,1-diphenyl-2-trinitrophenylhydrazine radical (DPPH•). The final concentration of the extract SC50 was compared. The results were as follows: 1.81 mg was extracted with coarse branches, followed by flower extraction of 1.235 mg, leaf extraction of 6.505 mg, and fine branch extraction of 8.486 mg. In the whitening in vitro experiment with mushroom tyrosinase, both of them have a significant effect on the inhibition reaction of L-Dopa. The MTT toxicity test was performed with 3T3 cells, showing that all extracted samples did not show any significant cytotoxicity at a final concentration of 5%. In the phototoxication test, flowers and leaves were also not significantly phototoxic. However, the stem and twigs are small but much lower than the vitamin C (below 10-4%). The repair test after cell scratching with 3T3 was carried out. Under the final concentration of 5%, the repair ability of the flower was more than twice that of the control group after 24 hours, which had a significant effect. In addition, the effect of the extract on UV-induced DNA damage was assessed using a DNA protection assay. The inhibitory effects of the four extracts on hydroxyl radical-induced DNA strand breaks were evaluated in a concentration-dependent manner by measuring the transformation of supercoiled pUC119 plastid DNA into a linear form. The order of DNA protection was from high to low: 72.9% of flowers, 66.7% of leaves, 40.9% of stem/coarse branches, and 26.8% of twigs. Based on the best activity of the flower extract, we use the flower extract, plus some other natural extracts and the necessary dosage additives to make the world's first friendly coral sunscreen without any chemical additives. And made a two-stage actual measurement of this product, obtained the following two significant evidences︰ First, it does protect E. coli. It survives completely without exposure to ultraviolet radiation for 30 minutes！The second is to test the PA and SPF values in vivo, and the results show that the PA value is up to ++++, and the SPF is also 40; if tested in vitro, the result shows that the SPF is 49.83, and the cw value is 381, Boots Star Rating is three stars. Product Results As we know in our experiments, the effect of UV protection is significant.