Nuciferine leaf polyphenol extracts and principal component Q3GA promotes proliferation in mice hippocampus and SH-SY5Y cell

• Main Authors: Sin-Rong Chen, 陳信融
• Other Authors: 王朝鐘
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/ev887f

碩士 === 中山醫學大學 === 生化微生物免疫研究所 === 106 === In the past, the laboratory found that the main component of lotus leaf polyphenols (NLPE) is Miquelianin (Q3GA). Foreign scholars have confirmed that Q3GA has the ability to promote neuronal cell proliferation in mouse hippocampus, and also promotes neural stem cell proliferation in vitro. Adult neurogenesis is the hope of improving aging and brain damage in the future. The purpose of this study was to evaluate the efficacy of lotus leaf polyphenols and its main component, Q3GA, in promoting neuronal proliferation and to be validated in vitro and in vivo. In this experiment, the extracted lotus leaf polyphenols were used for animal experiments. The lotus leaf polyphenols increased the hippocampal gyrus proliferation of C57BL/6 mice and increased the activation of Akt protein. Lotus leaf polyphenols also increase Cyclin A, E protein and reduce the performance of Cyclin D1. Both lotus leaf polyphenols and Q3GA can increase the proliferation of SH-SY5Y, while Q3GA can increase the protein expression of NGFR and PI3K/Akt pathway. Q3GA also increases Cyclin E protein and reduces the performance of Cyclin D1. The use of Akt1/2 kinase inhibitors to verify that p-Akt plays a role in cell proliferation. Based on the above experiments, we believe that lotus leaf extract has the ability to promote nerve cell proliferation, and lotus leaves are easy to grow in Taiwan. Therefore, the results of this study can be used to develop healthy foods that increase memory and prevent dementia, and hope to improve the impact of global aging.