The Correlation Between Phenotype and Genotype of Clinical Staphylococcus aureus Strains in Sterile Sites.

• Main Authors: Chi-Hao Chang, 張皓奇
• Other Authors: 曹世明
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/6g4c63

碩士 === 中山醫學大學 === 生化微生物免疫研究所 === 106 === Staphylococcus aureus is gram-positive cocci, frequently found in the nose, respiratory tract, and on the skin. Causes skin and soft tissue infection, bacteremia, pneumonia and other diseases with high mortality and complications., Methicillin-resistant Staphylococcus aureus is referred to as MRSA, and as known as “super bacteria” in common. MRSA is divided into two types according to the difference in phenotype ad genotype. The one acquired in hospital called “Hospital-acquired MRSA” (HA-MRSA), the other acquired in community called “Community-acquired MRSA” (CA-MRSA). They are mainly different in genotype. The purpose of studyaim to analyze the difference between phenotype and genotype of the MRSA isolated from the sterile sites. A total of 127 strains of MRSAwas collected and tested the antimicrobial susceptibilities for vancomycin, cefoxitin, and oxacillin by using agar dilution according to the guideline of Clinical Laboratory Standard Institute (CLSI). Phenotypes were detected by a minimum inhibitory concentration (MIC). Then, the genotype of the MRSA strain was detected by polymerase chain reaction (PCR). Results found that the detection of Phoenix is trusted . In genotype, there are some gaps and important findings. Whether manual or automatic detection of the instrument, there will be mistake. The gene of MRSA evolves with the environment, leading to the diversity of phenotype and geneotype. It is necessary to monitor changes of strains and carry out further research on genes in order to fit needs of clinical treatment.