Surface Modification of Polystyrene by Zwitterionic Self-Assembly for High-Throughput Diagnostics

• Main Authors: Rong-Xuan Xu, 許容瑄
• Other Authors: Yung Chang
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/c69pht

碩士 === 中原大學 === 化學工程研究所 === 106 === The development of nonfouling surfaces is therefore critically important in biomedical applications. Scientists began to develop zwitterionic antifouling materials based on the natural bio-inert theory that was inspired by the cell membrane[1, 2]. Despite their excellent fouling resistance, solubility issues of amphiphilic zwitterionic copolymers limit their use in surface modifications by coating[3]. This work presented an innovative method to form bio-inert interface. The strategy to create bio-inert interfaces in biological systems via hydrophobic interaction and ion -pair anchoring of zwitterionic copolymer brushes and a systematic study of how well-defined charges of the amphiphilic copolymers and zwitterionic copolymers and well -controlled surface of amp h ip h il i c co p ol ymer an d zwit t er io n i c co p o l ymer s aff ect b iocomp ati b il it y i s reported.Poly([2-(Methacryloyloxy)ethyl]trimethylammonium)- block -poly-(styrene) - block - poly([2-(Methacryloyloxy)-ethyl]trimethylammonium) (PTMA- b -PS- b -PTMA) with var ying h ydrophobic PS segment and pol y(3 -Sulfoprop yl methacr ylate) - block - poly(sulfobetaine methacrylate) (PSBMA- b -PSA) with varying negatively charged PSA lengths. via reverse radical addition-fragment chain transfer (RAFT).PTMA-b-PS-b-PTMA is coated on the polystyrene plate by hydrophobic interaction and PSA- b -PSBMA is coated on a surface covered with polycation brush via ion-pair anchoring. The advantages of the method are a very fast modification process and a modification without pretreatment. The results showed that the low fouling surface with respect to uncoated surface are achieved with shorter PS segment of triblock copolymer and shorter SA segment of diblock copolymer. Compare to PS Plate, the modified PS Plate has high sensitivity in blood typing. This kind of antifouling PS Plate has potential to develop high selectivity diagnosis in human blood.