| Summary: | 碩士 === 中原大學 === 化學研究所 === 106 === Metal-organic gels are constructed from metal ions or metal clusters and organic ligands to form permanent hierarchically porous structures such as micropores and mesopores through various non-covalent interactions. In recent years, the application of MOGs has been limited for gas storage, dye adsorption, catalysis, and chromatography. Since MOGs application for enzyme immobilization has not been reported, this study focused on the development of MOGs as an enzyme microreactor for starch hydrolysis. In the first part of this study, optimization on the synthesis and characterization of MOGs was carried out followed by enzyme immobilization via physical adsorption to form an enzyme microreactor. The second part of this study involves the optimization of the fabricated microreactor for starch hydrolysis. Based on the results, the enzyme microreactor prepared from aluminum based MOGs (AlBDC-8 and AlBDC-9) demonstrated the best performance for starch hydrolysis, which reaches up to 98%. Subsequently, a microreactor system was developed to simplify the reuse process of microreactors. Interestingly, the enzyme miroreactor system can be reused for at least 10 times with relative yield of 76%. This study demonstrated. The application of MOGs as enzyme support for the first time and applied in starch hydrolysis with high yield, high stability, ease of preparation and separation of products, suggesting its high potential for industrial application.
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