| Summary: | 碩士 === 朝陽科技大學 === 應用化學系生化科技碩博士班 === 106 === Raphanus sativus (radish), a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. SFE is a hydrolyzate form glucosinolates by myrosinase. Sulforaphene (SFE) and sulforaphane (SFN), two of bioactive constituents in radish, is an isothiocyanate that have a strong antioxidant compounds generally be found in Brassicaceae. In this study, the radish was pretreated and the contents of various parts were confirmed by HPLC. The highest content of SFE (48.171.11 μg/g) was found in the radish leaves, followed by the radish peeled root and the lowest is peel (0.480.05 μg/g). The SFE and its analogues, SFN, could be partially purified by liquid-liquid extraction and silica gel column chromatography. Since the SFE and SFN can not be directly identified by HPLC, two components were further confirmed by LC/MS. The unique functional groups -SCN for SFE/SFN were also confirmed by FTIR in the sample. The biological activity experiments found that the radish extract concentration of 600 μg/mL conditions, the clearance rate of DPPH 46.091.27%. Under SOD activity test, the inhibitory rate of SOD activity was 88.7% under the concentration of 1 mg/mL, the total activity of SOD was 426 U/g. ORAC values up to 1870 μM Trolox/g when the antioxidant capacity concentration at 10 μg/mL. These result showed that radish extracts have a very high antioxidant capacity. Furthermore, the inhibition zone size of 25.001.73 mm and 19.671.15 mm for Staphylococcus aureus and Salmonella enterica, respectively, was observed under agar diffusion method for radish extract at a concentration of 100 mg/mL. The results demonstrated that the radish extract has antibacterial activity against these two common pathogenic bacteria.
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