Anti-fatigue Effects of Chenopodium formosanum and Its Related Product

• Main Authors: LIN,MIN-YU, 林旻昱
• Other Authors: TSAI,MING-SHIUN
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/h2683b

碩士 === 大葉大學 === 生物產業科技學系 === 106 === Djulis (Chenopodium formosanum Koidz.) is a native crop in Taiwan. It has been called “Red pigweed” in Taiwan. It's a traditional crop by Taiwan aborigines cultivated for more than a century. In recent years, since its high nutritional value has been discovered, a large number of Djulis are now planted and it is developed to be a health food. The aim of this study is to investigate whether Djulis shows the anti-fatigue efficacy using a forced swimming mouse model. Mice were fed with fodder mixed with Djulis or a Djulis product, Djulis Yinbao Capsule (DYC). Six-week-old male C57BL/6 mice were divided into five groups: normal fodder fed group (control), fodder with low-dose DYC group, fodder with middle-dose DYC group, fodder with high-dose DYC group, and fodder with middle-dose Djulis group. After feeding for two weeks, mice in five different groups were examined their anti-fatigue efficacy by continuous swimming. The test methods are simplified by the methods for assessing the anti-fatigue efficacy of health foods announced by the Food and Drug Administration of the Ministry of Health and Welfare. They include forced swimming test, blood urea nitrogen test, and blood lactate test. Results of forced swimming test showed that mice fed with middle-dose Djulis and low- and middle-dose DYC showed significantly increasing times of swimming than mice in control group (p<0.001). Mice fed with high-dose DYC showed significantly increasing times of swimming than mice in control group (p<0.01). In brief, mice fed with fodder containing 3 different doses of DYC or middle-dose Djulis can all significantly elevate the forced swimming times than control mice. In blood urea nitrogen test, there was no significant difference between control and low-dose DYC groups, but showed significant differences in middle- and high-dose DYC and middle-dose Djulis groups when compared with control group (p<0.001). In brief, mice fed with fodder containing middle- or high-dose DYC or middle-dose Djulis can significantly reduce the concentrations of blood urea nitrogen after swimming. In the blood lactate test, the increments of blood lactate after swimming in both low- and middle-dose DYC were significantly lower than those in the control group (p<0.05), but there was no significant difference between high-dose DYC, middle-dose Djulis, and control groups. In brief, mice fed with fodder containing low- or middle-dose DYC can significantly reduce the concentrations of blood lactate after swimming. The reductions of blood lactate in mice after a 30-minute rest showed no significant difference between all tested group and control group. In summary, mice fed with fodder containing 3 different doses of DYC or middle-dose Djulis show anti-fatigue efficacy because of the increases of forced swimming times, reducing accumulations of blood urea nitrogen and lactate after swimming. Among all tested groups, middle dose of DYC is the most effective. Key Words: Djulis, Anti-fatigue, Forced swimming test, Blood urea nitrogen, Blood lactate.