The combined effect of vitamin C with low level-laser irradiation on melanoma in vitro and vivo

• Main Authors: Jui-Chi Lai, 賴瑞琦
• Other Authors: Li-Feng Liu
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/398w7z

碩士 === 義守大學 === 生物科技學系 === 106 === According to the data of the Top 10 cancer showed skin cancer is the eighth in the top ten cancer incidence rate of Taiwanese in 2015 by the Health Promotion Administration (HPA). Among the top five cancers with the fastest increase, it’s the second after the breast cancer. However, the main treatment of malignant melanoma is still use surgical resection now. Therefore, we are looking forward to find a non-invasive treatment for clinic. Previous studies have shown on high doses of vitamin C has the function of anti-tumor. And there are still many unknowns about the effects of low-energy lasers on cancer treatment. Therefore, we use these two treatments that have been used clinically to evaluate the effect on melanoma skin cancer growth. In this study, the data shown on (1) High concentration of vitamin C and low-energy laser can effectively reduce the survival rate and proliferation of tumor cells. (2) Decrease the phosphorylation of p-38 protein expression. (3) In animal experiments, the group with vitamin C combined with laser for 400 seconds (4J/cm2), the tumor growth rate was significantly lower than that of the control group. Our study shows that Vitamin C combines with low level-laser have a better effective for treat melanoma.