Anti-inflammatory effect of corylin on LPS-activated murine brain microglia cells

• Main Authors: Chia-En Tu, 凃嘉恩
• Other Authors: Chia-Yang Li
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/48j7q2

碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 106 === Background：Inflammation has been found to be associated with many neurodegenerative diseases, including parkinsonism and dementia. In the present study, we used lipopolysaccharide (LPS) to simulate murine microglia cells (BV2 cells) as an experimental model to mimic the inflammatory environment in brain. Aim: We used LPS-activated BV2 cells as an experimental model to examine the anti-inflammatory ability of corylin, a main compound isolated from Psoralea corylifolia L. which commonly used in Chinese herbal medicine. Method：The production of NO by LPS-activated BV2 cells was measured using Greiss reaction. The secretion of proinflammatory cytokines including tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) by LPS-activated BV2 cells was analyzed using ELISA. The expression of iNOS, COX-2, NLRP3, IL-1β and MAPKs in LPS-activated BV2 cells was examined by Western blot. Result：Our experimental results showed that corylin suppressed the production of proinflammatory cytokines by LPS-activated BV2 cells. In addition, corylin inhibited the expression levels of iNOS and COX-2, attenuated the phosphorylation of ERK, JNK and p38, inhibited the activation of caspase-1 and IL-1β, and decreased the expression levels of NLRP3 (Nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 OR Nod-like receptor protein 3) and ASC (Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain) by LPS-activated BV2 cells. These results indicate the anti-inflammatory effects of corylin may act through attenuating LPS-induced inflammation and inhibiting the activation of NLRP3 inflammasome in LPS-activated BV2 cells. Conclusion：Our experimental results suggest that corylin might have potential in treating brain inflammation and attenuating the progression of neurodegeneration diseases.