A Study on U-shape Optical Fiber Probe for Biomedical Sensing

• Main Authors: Yu-Le Li, 李宥樂
• Other Authors: Chia-Chin Chiang
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/nes988

碩士 === 國立高雄應用科技大學 === 機械工程系 === 106 === This study proposes a method for fabricating a U-shaped optical fiber probe sensor and applies it to biomedical measurements. The measured substances are glucose, miRNA-133a, SK-hep1, Mahlavu, etc. The sensing principle is influenced by the external medium by the optical whispering gallery mode (WGM). It is affected by the refractive index of the external environment, and the interference changes with the core layer and the shell layer. The change of wavelength will occur drift and transmission loss will increase or decrease. In this study, U-shaped and spiral U-shaped optical fiber probe sensors are fabricated by miniature-fiber firing method. The bending radius of the spiral U-shape is divided into 0.6 mm and 0.75 mm. In the sensing aspect, the spiral U-shaped optical fiber probe sensor has no coated sensing layer to measure the glucose solution. The experimental results show that the sensor measurement spectrum is stable when the number of knots is double. The smaller the bending radius, the higher the sensitivity of transmission loss. The spiral U-shaped optical fiber probe sensors has a fixed spiral shape, so the radius will not change. It can achieve packaging effects similarly and reduce packaging process steps. The U-shaped optical fiber probe sensor is coated the sensing layers with nano-particles, glucose oxidase and miRNA-133a probe for measurement. The results of coated glucose oxidase sensing layer for glucose measurement showed good sensitivity and linearity. The wavelength sensitivity is 2.8992 nm/% and the linear regression R2 is 0.9771. The wavelength sensitivity is 5.15 times higher than uncoated sensing layer. The results of measuring miRNA-133a showed good sensitivity and linearity. The wavelength sensitivity is 0.022 nm/nM and the linear regression R2 is 0.896. The lowest concentration limit is 0.5 nM. Measuring Mahlavu with SK-hep1 as a comparison group also showed good sensitivity and linearity. The wavelength sensitivity is -763.916 nm/(ng/ml) and the linear regression R2 is 0.989. The lowest concentration limit is 0.0133 ng/ml. From the above description, it can be confirmed that U-shaped optical fiber probe sensor of this study not only has excellent performance in biomedical measurement, but also has many advantages such as light weight, small volume, high sensitivity and linearity. In the future, multi-sensors can be connected in series by using split-wave multiplexing technology, and measuring the unknown samples. I hope that can provide a reference for medical personnel.