Genome-wide association study for egg quality and feeding traits of Taiwan Country chicken

• Main Authors: Kai-Hsiang Lin, 林楷翔
• Other Authors: 陳志峰
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/5t3wnr

碩士 === 國立中興大學 === 動物科學系所 === 106 === A genome-wide association study (GWAS) used a high density genotyping platform represents a method for identifying genetic variations influencing various traits, and the GWAS is the newest tool being proposed to the poultry breeding industry for improvement of animal agricultural species. The object of this study was found the significant and suggestive single nucleotide polymorphism (SNP) which should influence the traits of the egg production, egg quality and the relevant of the feeding traits in our native chicken population. We used the F2 population of 743 individuals produced by crossing the Taiwan country chicken L2 line with and experimental line of Rhode Island Red layer R- provided by INRA in 2003.Total of 22 traits were analyzed which including egg number (EN), age at the first egg (AFE), laying rate (LR), egg weight (EW), egg color (EC), egg length (egg_L), egg width (egg_W), egg index (egg_I), eggshell strength (ES), yolk weight (YW), albumin height (AH), eggshell within membrane (EWM), eggshell without membrane (EOM), eggshell membrane thickness (EMT), eggshell weight (ESW), Average feed intake (AFI), Average daily gain (ADG), eggmass, feed efficiency (FE), Residual feed intake (RFI). There are three ways of the statistical analysis were used, including generally linear model (GLM) in software of plink, linear mixed model (LMM) and Bayesian sparse linear mixed model (BSLMM) in software of GEMMA. The results showed that the rs15037617 associated with AFE and GGaluGA165228 associated with EN and LR. There are 14 SNPs were found in LMM associated with 7 traits in egg quality. The traits of egg colour a* have three SNPs associated with rs15409520, rs13593225, rs14637358; egg index associated with rs15190652; ES associated with two SNPs including GGaluGA320913 and GGaluGA007314; there are two SNPs were found, s14075934、GaluGA235913, associated with yolk weight; the SNPs GaluGA134929 and GaluGA006669 were found associated with the traits EWM and EMT, respectively; there are four SNPs were found associated with ESW, including s10721950, GGaluGA082982, rs13764758, GGaluGA264151. There are five SNPs we found that associated with three feeding traits, including AFI, eggmasss and RFI. In the traits of AFI, we found two SNPs including GGaluGA114124 and rs13841376; there are also two SNPs associated with eggmass which named rs15008789 and GGaluGA107494; there are one SNP, GGaluGA020046, we found that associated with RFI. In the analysis of the BSLMM, we found the associated SNPs in egg quality traits only. The trait of egg colour L* associated with GGaluGA000698 and rs14099457; the egg colour a* was associated with rs13746326, rs15409520 and GGaluGA000698; the ESW was associated with rs14480552, rs13764758 and GGaluGA082982. In conclusion of this study, the SNPs may be improved the poultry breeding program rapidly.