The studies of plantlet for Lilium callosum and seedling cultivation for Lilium formosanum

• Main Authors: Sunya Poompoung, 澎山亞
• Other Authors: Chen Chang
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/zma5df

碩士 === 國立中興大學 === 園藝學系所 === 106 === Abstract Lilium callosum Sieb. et Zucc. and Lilium formosanum wall. are Lilium species native to Taiwan. In this study, we explored for improving the cultivation technique of these two Lilium species. We investigated the effects of bulb scale size on the bulblet proliferation in vitro and plantlet growth in greenhouse. The result showed the scale of the large size (above 9.0 mm width) formed the maximum bulblet number per scale (2.3 bulbs). However, the survival rate, leaf number and root number per scale and the morphogenesis was non significantly different in explants of different scale size. Three cytokinins (BA, kinetin and TDZ) of 0.01, 0.1 and 1 mg/l were applied on scale of above 9 mm size for evaluating the bulblet proliferation. Callus was induced at the highest concentration on the three type of cytokinins after 30 days of culture. Scale explants of Lilium callosum in the treatment of 0.1 mg/l TDZ formed the height number of bulblets (4.7 bulbs). Three different size of bulblets, after low-temperature stratification for 2 months and followed with cultivation in the greenhouse for 4 months. The results showed that the highest survival rates of 60%, bulblet size and fresh weight 1.6 g were obtained with bulblet of size 10.0-12.0 mm in diameter. The maximum diameter of bulblet, leaf number, leaf length and root length were obtained in the treatment of 1.5 g/l fertilizer input. The results of acclimatization of Lilium callosum showed non-significant difference on the number of leaves, the width of bulblet, and number of root in four kinds of mixed media. Lilium callosum on plant media contained peat moss (BVB): perlite (1:3) showed the highest of the number of leaves (2.8 leaves), bulblet size: length (18.6 mm) and width (8.7 mm), root length (6.4 cm) and whole plant weight (0.6 g). In conclusion, peat moss (BVB): perlite (1:3) had the maximum potential for growth characteristics on Lilium callosum. The result of Seedling growth on mixed media of Lilium formosanum (L500S and L507S) showed after 5℃ stratification for 1 month, there is a significant difference in germination percentages between L507S (99%) and L500S (91%). After 4 months of cultivation on mixed media for acclimatization in greenhouse, the results indicated that L507S seedlings had the highest elongation rate (50%), number of leaves (11 leaves) and leaf length (26 cm) with the composition of peat moss (Ke): perlite: vermiculite: rice husk ash (2:1:1:0.5). The first flower buds were observed after 17 months of cultivation, and the highest formation rate of flower buds was obtained in L507S (41.7%) and L500S (25.0%) on peat moss (Ke): perlite: vermiculite: rice husk ash (2:1:1:0.5). Therefore, the optimal composition of mixed media for Lilium cultivation should possess the properties of aeration porosity, water holding capacity. The conservation of endemic Lilium species is invaluable, and we applied in vitro culture to overcome the reproductive barrier and establish the cultivation technique in the greenhouse