Novel Fluorescent Nanoparticles Constructed from AIEgens

• Main Authors: Yi-Hsuan Chen, 陳依瑄
• Other Authors: Cheng-Chung Chang
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/fj88va

碩士 === 國立中興大學 === 生醫工程研究所 === 106 === The major goal for our research is to use simple synthesized method for various fluorescence dye derivatives containing acridine core via Heck reaction and Suzuki coupling reaction. The basic optical properties of acridine derivatives and their effects in acidic and alkaline environments were investigated. It is more important to study whether they have the characteristics of Aggregation-Induced Emission (AIE). We will molecules with AIE properties and capable of forming FONs, which are directly coated by the Stöber method to form dye-doped silica nanoparticles (DDSN). A set of amino acid fluorescence turn-on sensor-based fluorescence organic nanoparticles ASP@OH and CSP@OH were constructed by the modified stöber method. The constructed procedure can be divided into two steps. The room temperature-reacted amino acid sensors were firstly silanized with DDSN precursor in an ethanol solution. This is the first stage of emission enhancement due to intramolecular charge transfer (ICT) or photo-induced electron transfer (PET). After that, silane was further added to coat on the core to form a silica shell. The fluorescence of the obtained ASP@OH and CSP@OH is expected to increase further with AIE properties. That is, the ICT-induced fluorescence enhancement phenomenon was promoted according to the AIE effect that can be used to build-up a novel double emission enhancement (DEE) platform. Systematic investigations verify that the resulting ASP@OH and CSP@OH could display extremely conservative, solvent-independent and photo-stability optical properties in aqueous media as well as in most organic solvents. It means that these novel fluorescent nanoparticles are suitable to be a biomarker of living cells and functioned as fluorescent visualizers for intracellular imaging.