Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells
碩士 === 國立交通大學 === 應用化學系分子科學碩博士班 === 106 === In this thesis, fluorescent probe NBDMP is designed and synthesized to detect biothiols Cys, Hcy, GSH. The fluorescent probe NBDMP consisted of two fluorophores NBD and (E)–4-(4–hydroxystyryl)-1-methyl pyridinium iodide. NBD is a fluorophore and also a rea...
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ndltd-TW-106NCTU53090012019-05-16T00:08:11Z http://ndltd.ncl.edu.tw/handle/f7hc5e Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells 硫醇螢光探針之設計與合成及活體細胞的應用 Tseng, Ying-Ling 曾映綾 碩士 國立交通大學 應用化學系分子科學碩博士班 106 In this thesis, fluorescent probe NBDMP is designed and synthesized to detect biothiols Cys, Hcy, GSH. The fluorescent probe NBDMP consisted of two fluorophores NBD and (E)–4-(4–hydroxystyryl)-1-methyl pyridinium iodide. NBD is a fluorophore and also a reaction part for thiol. NBDMP reacts with Cys, Hcy, GSH to obtain 10-fold green fluorescent enhancement from pyridinium fluorophore and 65-fold and 110-fold yellow fluorescent enhancement from NBD fluorophore by Cys and Hcy due to intramolecular sulfur-amine rearrangement in NBD fluorophore. NMDMP also shows good selectivity to distinguish Cys/Hcy from GSH. The detection limit of Cys, Hcy and GSH are 73 nM, 51nM and 128 nM, respectively and can be used in biothiol imaging. Wu, Shu-Pao 吳淑褓 2017 學位論文 ; thesis 52 en_US |
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碩士 === 國立交通大學 === 應用化學系分子科學碩博士班 === 106 === In this thesis, fluorescent probe NBDMP is designed and synthesized to detect biothiols Cys, Hcy, GSH.
The fluorescent probe NBDMP consisted of two fluorophores NBD and (E)–4-(4–hydroxystyryl)-1-methyl pyridinium iodide. NBD is a fluorophore and also a reaction part for thiol. NBDMP reacts with Cys, Hcy, GSH to obtain 10-fold green fluorescent enhancement from pyridinium fluorophore and 65-fold and 110-fold yellow fluorescent enhancement from NBD fluorophore by Cys and Hcy due to intramolecular sulfur-amine rearrangement in NBD fluorophore. NMDMP also shows good selectivity to distinguish Cys/Hcy from GSH. The detection limit of Cys, Hcy and GSH are 73 nM, 51nM and 128 nM, respectively and can be used in biothiol imaging.
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Wu, Shu-Pao |
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Wu, Shu-Pao Tseng, Ying-Ling 曾映綾 |
author |
Tseng, Ying-Ling 曾映綾 |
spellingShingle |
Tseng, Ying-Ling 曾映綾 Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells |
author_sort |
Tseng, Ying-Ling |
title |
Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells |
title_short |
Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells |
title_full |
Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells |
title_fullStr |
Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells |
title_full_unstemmed |
Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells |
title_sort |
design and synthesis of fluorescent probe for thiol and its applications in living cells |
publishDate |
2017 |
url |
http://ndltd.ncl.edu.tw/handle/f7hc5e |
work_keys_str_mv |
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1719161685959245824 |