Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells

• Main Authors: Tseng, Ying-Ling, 曾映綾
• Other Authors: Wu, Shu-Pao
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/f7hc5e

碩士 === 國立交通大學 === 應用化學系分子科學碩博士班 === 106 === In this thesis, fluorescent probe NBDMP is designed and synthesized to detect biothiols Cys, Hcy, GSH. The fluorescent probe NBDMP consisted of two fluorophores NBD and (E)–4-(4–hydroxystyryl)-1-methyl pyridinium iodide. NBD is a fluorophore and also a reaction part for thiol. NBDMP reacts with Cys, Hcy, GSH to obtain 10-fold green fluorescent enhancement from pyridinium fluorophore and 65-fold and 110-fold yellow fluorescent enhancement from NBD fluorophore by Cys and Hcy due to intramolecular sulfur-amine rearrangement in NBD fluorophore. NMDMP also shows good selectivity to distinguish Cys/Hcy from GSH. The detection limit of Cys, Hcy and GSH are 73 nM, 51nM and 128 nM, respectively and can be used in biothiol imaging.