Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test

• Main Authors: Wei, Lan-Yi, 魏蘭懿
• Other Authors: Yuan, Chiun-Jye
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/x7u84u

碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 106 === Protein tyrosine kinases are highly related to many human diseases, including neuron degenerative diseases, autoimmunity, diabetes, and cancers. Previously, a tyrosinase-based tyrosine kinase biosensor was developed and characterized by using Src as a model in our lab. In this study two other tyrosine kinases, Hck and Her2, were further explored and studied on a novel electrochemical detection setup with the previously developed tyrosine kinase sensing protocol. Hck protein kinase has been shown to be related to various immune disorders, such as chronic myelogenous leukemia, multiple myeloma and acute lymphatic leukemia. The dysfunction of Her2 protein kinase is strongly associated with the pathogenesis of various human cancers, including non-small cell lung cancer, ovarian cancer, pancreatic cancer, endometrial cancer and colon cancer. The platform allows reaction to be carried out in a small sample and with low noise. The results showed that the developed detection platform could be used for protein tyrosine kinases activity assay and for drug screening of these protein tyrosine kinases.