Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test
碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 106 === Protein tyrosine kinases are highly related to many human diseases, including neuron degenerative diseases, autoimmunity, diabetes, and cancers. Previously, a tyrosinase-based tyrosine kinase biosensor was developed and characterized by using Src as a model...
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ndltd-TW-106NCTU55380082019-05-16T00:22:51Z http://ndltd.ncl.edu.tw/handle/x7u84u Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test 酪胺酸蛋白激酶活性生物感測平台的開發與在抗癌藥物測試上的應用 Wei, Lan-Yi 魏蘭懿 碩士 國立交通大學 分子醫學與生物工程研究所 106 Protein tyrosine kinases are highly related to many human diseases, including neuron degenerative diseases, autoimmunity, diabetes, and cancers. Previously, a tyrosinase-based tyrosine kinase biosensor was developed and characterized by using Src as a model in our lab. In this study two other tyrosine kinases, Hck and Her2, were further explored and studied on a novel electrochemical detection setup with the previously developed tyrosine kinase sensing protocol. Hck protein kinase has been shown to be related to various immune disorders, such as chronic myelogenous leukemia, multiple myeloma and acute lymphatic leukemia. The dysfunction of Her2 protein kinase is strongly associated with the pathogenesis of various human cancers, including non-small cell lung cancer, ovarian cancer, pancreatic cancer, endometrial cancer and colon cancer. The platform allows reaction to be carried out in a small sample and with low noise. The results showed that the developed detection platform could be used for protein tyrosine kinases activity assay and for drug screening of these protein tyrosine kinases. Yuan, Chiun-Jye 袁俊傑 2017 學位論文 ; thesis 44 en_US |
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碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 106 === Protein tyrosine kinases are highly related to many human diseases, including neuron degenerative diseases, autoimmunity, diabetes, and cancers. Previously, a tyrosinase-based tyrosine kinase biosensor was developed and characterized by using Src as a model in our lab. In this study two other tyrosine kinases, Hck and Her2, were further explored and studied on a novel electrochemical detection setup with the previously developed tyrosine kinase sensing protocol. Hck protein kinase has been shown to be related to various immune disorders, such as chronic myelogenous leukemia, multiple myeloma and acute lymphatic leukemia. The dysfunction of Her2 protein kinase is strongly associated with the pathogenesis of various human cancers, including non-small cell lung cancer, ovarian cancer, pancreatic cancer, endometrial cancer and colon cancer. The platform allows reaction to be carried out in a small sample and with low noise. The results showed that the developed detection platform could be used for protein tyrosine kinases activity assay and for drug screening of these protein tyrosine kinases.
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author2 |
Yuan, Chiun-Jye |
author_facet |
Yuan, Chiun-Jye Wei, Lan-Yi 魏蘭懿 |
author |
Wei, Lan-Yi 魏蘭懿 |
spellingShingle |
Wei, Lan-Yi 魏蘭懿 Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test |
author_sort |
Wei, Lan-Yi |
title |
Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test |
title_short |
Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test |
title_full |
Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test |
title_fullStr |
Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test |
title_full_unstemmed |
Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test |
title_sort |
development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test |
publishDate |
2017 |
url |
http://ndltd.ncl.edu.tw/handle/x7u84u |
work_keys_str_mv |
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