| Summary: | 碩士 === 國立嘉義大學 === 農藝學系研究所 === 106 === In this study, the genes regulated by OsCDPK1 were isolated via transcriptome analysis. Among which one gene is identified as OsGF14c (Oryza sativa G-box binding factor, a 14-3-3 protein). Northern blot hybridization showed that the expression of OsGF14c is abundantly in the germinating seed, mature and senescent leaves, and induced in the OsCDPK1-Ox seedlings under drought stress. The rice seedlings are shorter and seeds are smaller in OsGF14c-Ox than in WT. Whereas the OsGF14c-Ox plants confer increasing tolerance on drought and salt stress than that of WT. These results demonstrated that OsGF14c play roles that negatively affected on plant growth and development, but positively on the improvement of salt and drought stresses tolerance. In Arabidopsis, a bushy mutant bus3 was isolated from the population of the activation T-DNA tagging mutants. The T-DNA insertion site was identified by plasmid rescue and found that the targeted site was located on the chromosome 2 of an unknown function gene, named as UnP2. Ectopic expression of UnP2 (UnP2-Ox) in Arabidopsis revealed that the transgenic plants exhibited delayed flowering time and senescence.
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