Preparation of CRGDS Pentapeptide Derivative as a Biosensor for the Specific Recognition of Cancer Cell Receptor

• Main Authors: Yu-Hua Lin, 林鈺樺
• Other Authors: Chien-Chung Cheng
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/w85ts4

碩士 === 國立嘉義大學 === 應用化學系研究所 === 106 === Arg-Gly-Asp (RGD) peptide-functionalized gold nanoparticles which can be taken up by αvβ3 integrin-expressing cell lines has been reported by Shukla et al.[5] The αvβ3 integrin is an important marker of the neovasculature and is normally found during tumour angiogenesis; without it, tumours cannot grow beyond 1-2 mm in size.[5] Therefore, αvβ3 integrin is relatively important for the identification of tumour cells by CRGDS pentapeptide. In this thesis, preparation of CRGDS pentapeptide for the specific recognition of cancer cell receptor by solid phase peptide synthesizer had been successfully. However, the linear CRGDS pentapeptide was easily degraded by biodegradable enzymes, so the SH group on the cysteine of CRGDS coordinates with the gold nanoparticles not only to form stable complexes with covalent bonds but also to enhance the stability of the nanoparticle system in vivo. In addition, the large size AuNP@CRGDS entered the organism, which had anchored and become adhered to the αvβ3 integrin, so it could be used as a specific identification biosensor for cancer cell receptors. However AuNP@CRGDS derivatives did not have light-emitting properties and were not easily tracked in vivo. To deal with above, AuNP@CRGDS was modified with fluorescent molecules or changed into gold clusters and then modified anticancer drug, which can be quickly observed, tracked and with the function of drug carrier. Therefore, the main purpose of this thesis was to prepare AuNP@CRGDS as the foundation for the future restructuring or modification of other functional group molecules. Finally, NMR and FTIR were used to identify the structure and functional group of CRGDS pentapeptide. And its derivative AuNP@CRGDS was determined and identified by FTIR, UV-Vis spectrometer, DLS and AFM.