Immunomodulatory Properties of Phosphoglycolipids

• Main Authors: WEI, CHIH-FENG, 魏志峰
• Other Authors: PENG, YI-REN
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/78qaj8

碩士 === 國防醫學院 === 病理及寄生蟲學研究所 === 106 === Cytokine production from activated macrophages activates the immune system and increases the host defense against invading pathogens. However, aberrant cytokine production is harmful to the cells and tissues, which lead to the pathogenesis of inflammatory diseases. Therefore, it is important for the host to regulate the immune response precisely. Our previous studies showed that phosphoglycolipids (PGL) isolated from the thermophilic bacteria exhibit immune modulation properties; however, the structure-activity relationship is unclear. The aim of the present study was to investigate the immune modulation potential of synthetic structural defined PGL and possible underlying molecular mechanisms. After evaluation the effect of 19 synthetic PGL on interlukin-6 (IL-6) production in lipopolysaccharide (LPS)-activated macrophages, we found several PGL reduced the IL-6 production, being PGL #5 is more potent than others. Interestingly, in the same condition, PGL #5 increased the tumor necrosis factor-α (TNF-α) production. These results indicate that PGL #5 had bi-functional effects on LPS-activated macrophages. In addition, the mechanistic analysis revealed that PGL #5 enhanced the nuclear factor-kappaB (NF-κB) activation in LPS-activated macrophages, while PGL #5 alone did not affect NF-κB activation. However, PGL #5 had no effects on the production of reactive oxygen species (ROS) and the phosphorylation of ERK1/2, JNK1/2 and p38. Our current results indicated that PGL #5 increased LPS-mediated TNF-α production may through enhanced NF-κB activation; however, how PGL #5 reduced IL-6 production needs further investigation. Taken together, PGL had immune modulation properties and had the potential to be used in the development of anti-inflammatory agent and adjuvant.