| Summary: | 碩士 === 國立屏東科技大學 === 生物科技系 === 106 === Metarhizium anisopliae is a kind of entomopathogenic fungi on insects, it is distributed all over the world. The main application for this fungi is as a pest control for crops, acting as a biological pesticide. In previous studies, Metarhizium anisopliae was able to produce a large quantity of secondary metabolites containing anticancer and antiviral activities. In this study, Metarhizium anisopliae MA-126 was cultured into solid-state fermentation and liquid state fermentation. After partition extraction, the NS2B protein had 35% and 10% inhibition rate when both of them have 50μg/ml concentration on ethyl acetate layer. The ethyl acetate layer was purified by LH-20 column, high performance liquid chromatography (HPLC) to yield eight compounds. Compound1-4 are separation from liquid state fermentation(5S,8S,9R)-8-benzoyl-9-hydroxy-8-methoxy-3-methyl-2-(5-methylfuran-2-yl)-1-oxa-7-azaspiro[4.4]non-2-ene-4,6-dione(1), (3S,3aR,7aS)-3a,7a-dihydroxy-3-((S)-1'-hydroxy-4'-methylpent-3'-en-1'-yl)-4-methoxy-3-methylhexahydroisobenzofuran-5(3H)-(2), chlovalicin(3), (4S,4aR,5S,8aR)-4,4a,8a-trihydroxy-5-methoxy-4-methyl-3-(3'-methylbut-2'-en-1'-yl)octahydro-6H-isothiochromen-6-one(4), compound 5-8 are separation from solid state fermentation. Destruxin A(5), Destruxin B(6), Destruxin E chlorohydrin(7) and Destruxin E diol(8).
All structures were elucidated by NMR spectra techniques, including 1H, 13C, DEPT, COSY, HMQC, HMBC, and NOESY, with physical data(mass spectrometry, optical rotation, IR spectrometry), also compare with the compound data in the literature to establish.Compound 1 is the new compound, compound 1-4 are the first natural compounds isolated from Metarhizium anisopliae.
|
|---|
