| Summary: | 碩士 === 國立屏東科技大學 === 動物疫苗科技研究所 === 106 === Pigeon Circovirus (PiCV) has been reported worldwide ever since it was reported in the 1980s. The virus attacks the pigeons' immune system specifically the bursa, which can cause immunosuppression and subsequently lead to secondary bacterial, viral or protozoan infections. PiCV infects young and old pigeons, but easily found in four to six months old pigeons therefore it is also known as young pigeon disease syndrome (YPDS). Virus-like particles (VLPs) are produced by the self-assembly of structural proteins of viruses. It is similar to natural viruses but without the viral genome. Compared with the traditional subunit vaccine, the VLP vaccine can be more effectively recognized by the antigen-presenting cells and induce a stronger immune response. In this study, the PiCV capsid protein gene was cloned into the pET32a and pGS21a vectors. Escherichia coli expression system was used to produce the recombinant PiCV capsid protein. The capsid protein will assemble into VLPs. Following purification, western blot analysis was used to confirm the antigenicity. Then, vaccine was injected via subcutaneous injection. Western blot assay showed significant band at greater than 180 k Da thus, it was hypothesized that it is a virus-like particle. Soluble protein production in pET32a-picv is better than in pGS21-picv. Induced PiCV capsid protein, more VLPs assembled at 30°C than in 37°C. The animal test showed the antibody titer of immunized with VLPs is higher than the control group. After the animal boost, they would have faster and stronger immune response.
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