| Summary: | 碩士 === 國立屏東科技大學 === 獸醫學系所 === 106 === In Taiwan, Disease Investigation of parrot rarely mentioned to proventricular dilatation disease (PDD) caused by avian bornaviruses. Its characteristic tissue lesions are lymphocytic-cytoplasmic peri-intestinal peripheral ganglionitis and encephalomyelitis, which can also be referred to as avian bornaviral ganglioneuritis (ABG). In this paper, we focused on the investigation of avian bornaviruses for vomit samples from 240 parrots in Taiwan, and analyze the sequence of 6 complete cases. Our laboratory examination including RT-PCR for the detection of pathogens and gene sequencing in 6 intact cases, to compare M gene mutations and observe the tissue section lesions. Six of the complete cases, including Guaruba guarouba, Ara ararauna, and Psittacus erithacus etc. that are positive reaction and these cases all have PDD. The sequence alignment of PC14-460 showed a point mutation in the 97th nucleotide; the PC14-466 and PC15-483 sequence fragments had point mutations in 52, 64, 151, 187, 199, 252 nucleotides; the cases PC14-466 and PC15-483 were the 252nd nucleotide mutation point, resulting in the translation of the amino acid changes, from the original lysine to arginine. A total of 240 samples were collected for vomiting, the frequency of the Bonavirus was 19.6%. Percentage of all ages, parrots accounted for 4.2% from 1 to 6 months, parrots from 6 months to 3 years is 5.8%, from 3 to 7 years is 4.6%, and from 7 to 30 years is 5.0%.The seasonal distribution ranges from 4.2% in February to April (spring), 5.8% in May to July (summer), 13.3% in August to October (fall), November to January (winter) Accounted for 11.7%. The frequency of Bonavirus in the breeding ground is high. The proportion of Bonavirus in each parrot species was 21% in Psittacus erithacus, 17.9% in Ara ararauna, 26.2% in Guaruba guarouba, 15.6% in Cockatoo, and 16.7% in other parrot species.
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