| Summary: | 碩士 === 國立臺灣師範大學 === 化學系 === 106 === In this study, we scaled up fermentation volume from 1 mL to 20 L. Gas chromatography - mass spectrometry was used for the detection of ethanol concentration. A novel technology of using microtube array membranes (MTAM) immobilized yeast cells for mass production of ethanol fermentation. Two types of MTAMs were used to conduct the experiment namely, renewable poly-L-lactic acid (PLLA) and polysulfone (PSF). The MTAMs were cut into a 1 cm (width) x 3 cm (length) size, and the yeasts were siphoned at the desired concentration into the respective MTAMs The presence of nanopores on the ultrathin surface of the MTAM allows for the efficient diffusion of nutrient and waste across the lumen wall. The encapsulated yeast reduced the inhibitory effects, while significantly reducing the cost by repeated use of the encapsulated yeast and the reduction of the need for costly separation. Compared to free cells, MTAM encapsulated yeast registered a better glucose to ethanol conversion efficiency; and in turn registered a higher ethanol concentration of 15% after 10-14 days of fermentation. The successful scaled up fermentation demonstration suggested that potentially, the encapsulation technical using the MTAMs can be applied to various fermentations as a solution to increase output without major modification to existing infrastructures.
|
|---|
