Effects of exercise on liver function through inhibition of NF-κB inflammatory signaling pathway in diabetic db/db mice

• Main Authors: Wu, Ci-Hang, 吳啟行
• Other Authors: Liu, Hung-Wen
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/x62xm2

碩士 === 國立臺灣師範大學 === 體育學系 === 106 === Hepatic lipid accumulation induced by metabolic dysfunction was accompanied with type II diabetes. Increased intrahepatic lipid content triggers oxidative stress, thus activating IκB-α/NF-κB pathway. Furthermore, activation of the inflammatory pathway and increases in circulating TNF-α and IL-6 levels cause liver damage. The purpose of this study was to determine whether exercise training upregulates the energy metabolism pathway and inhibits inflammatory pathway, thereby alleviating hepatic lipid accumulation and injury. Methods: Eight weeks moderate-intensity exercise (5m/min, 60min/day, and 5days/week for a total of 8weeks) on the Sirt1/AMPK-α/PGC1-α axis and NF-B pathway were investigated in liver of diabetic db/db (C57BL/KsJ-leprdb/leprdb) mice. The statistical significance of the differences between two groups was determined by independent sample t-test (P < 0.05). Results Upregulation of SIRT1 and PGC1-α expression and AMPK activity were observed in db/db mice with exercise training. Decreases in phosphorylation of IB and NF-B were observed in db/db mice with training compared with untrained mice. Conclusion Aerobic exercise training inhibits NFB signaling pathway and activates SIRT1/AMPK pathway, thereby alleviating hepatic lipid accumulation and injury.