Transcriptome analysis reveal effect of health promotion on metabolic-related gene expression in obesity

• Main Authors: Jacqueline Ho Jia Lin, 何佳霖
• Other Authors: Lu, Ming-Wei
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/37d437

碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 106 === Metabolic syndrome (MetS) is a biological alert which signals medical disorders that are tightly associated with morbidity and mortality of cardiovascular diseases in contemporary society. People adjust lifestyle patterns can not only improve their health conditions and quality of life, but also help to prevent the diseases caused by metabolic syndrome, which include diabetes mellitus, hypertension, and dyslipidemia. In order to analyze the influence of health promotion activities on genes related to metabolic syndrome in obesity systematically, we will investigate the mechanism by transcriptome analysis. In this study, blood tests of four adults having metabolic syndrome were done before and after health promotion. Using RNA-seq, genes sequencing and mapping were analyzed to calculate the differential gene expression and forward the gene with significant differential expression for further study. The result showed that most of the parameters were improved after joining health promotion, except FBS, HDL and strength and endurance while BF is the only parameters with significant changes (p<0.05). After sequencing by NGS, the gene expressions were calculated and filtered by p<0.05 as well as logFC>1. We found 8 genes that were involed in insulin signaling pathway. Among them, PDE3, AKT and mTOR was significantly upregulated whereas aPKC, p70S6K, PYG, BAD and Ras had shown a significant downregulation in insulin signaling pathway. The activation of insulin signaling pathway could increase glucose uptake and balance blood glucose level to avoid Mets and T2DM. In the present study, we explored and analyzed the expression of metabolic related genes through health promotion activities, without weight reducing drug or surgery and provided the evidence for the mechanism of health promotion activities on genes related to metabolic syndrome in obesity. Keyword：Metabolic syndrome, Health promotion, RNA-seq