| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 106 === Tripneustes gratilla is a common sea urchin in Taiwan but its edible part is only about 10% of the total weight. Crassostrea gigas is the most popular oyster in Taiwan but its edible part is only about 30% of the total weight. The purpose of this study was to use different formula of shell powder from sea urchin T. gratilla and oyster C. gigas for converting to magnesium substituted β-tricalcium phosphate (β-TCMP) as the feasibility of bone biomaterials.
After the assays of X-ray diffraction, fourier transmission infrared spectrometry, scanning electron microscopy, and energy dispersive X-ray spectrometry, all materials of different formula of shell powder from sea urchin T. gratilla and oyster C. gigas, are successfully converted to β-TCMP and hydroxyapatite (HA). Among them, only sea urchin T. gratilla group can be converted to the bone graft material β-TCMP. Furthermore, oyster shell C. gigas with different dose of MgCl2·6H2O / SrCl2·6H2O can be also converted to the bone graft material β-TCMP by hydrothermal reaction.
Then osteoblast MG-63 cells were cultured in soaking solution with bone graft biomaterials by using MTT assay to determine the cell viability. Otherwise, osteoblast MG-63 cells were cocultured with those bone graft biomaterial tablest, by using MTT assay to determine the cell viability. The result revealed that all bone graft biomaterials and those tablets were non-toxic. Therefore, the β-TCMP was found from using urchin shell only and oyster shell with higher dose of MgCl2·6H2O and SrCl2·6H2O than sea urchin shell. Those bone graft biomaterials formed from different formula of sea urchin and oyster shell, showed HA structure.
The bone graft biomaterials from sea urchin shell and oyster shell with MgCl2·6H2O / SrCl2·6H2O supplement exhibited to increase MG-63 cell growth, especially for 2 folds of dose of MgCl2·6H2O / SrCl2·6H2O in the oyster shell.
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