Hyaluronidase-responsive drug delivery system with dual-imaging and dual-target function by using mesoporous silica nanoparticles

• Main Authors: Wu, Zhi-Yuan, 吳智源
• Other Authors: 林秀美
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/8xwzq2

碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 106 === Nanoparticle-based drug delivery systems are the most popular research topic in recent years, Compared with traditional drug carriers, mesoporous silica nanoparticles (MSN) has good surface modifiable ability, adjustable pore size and has good biocompatibility. MSN had become the research direction of most scientists. In this study, MSN-EuGd was synthesized by incorporating Eu3+ and Gd3 + during the synthesis of MSN. Then the surface of the material was connected to the TAT peptide of the nucleus of the target cancer cell and then loaded with the anticancer drug camptothecin CPT). Then, the surface of MSN-EuGd was bonded to the hyaluronic acid as active target and gatekeeper。With this system, it is desirable to achieve dual imaging, dual targeting, and to deliver drug to the cell nucleus at a Hyaluronidases controlled release. The experiment is divided into three parts. First, we make the material have fluorescent and magnetic dual-imaging property by incorporating Eu3+ and Gd3 + into the MSN. The second part is modified the cell membrane target molecule and the nucleus target molecule on the surface of the nanoparticle, making the nanoparticle a target drug carrier. The third part is the loading of drug molecules into the carrier, giving the entire carrier a specific target profile and the ability to treat cancer. In this study, we investigated the basic properties of the drug carrier, such as physical properties and chemical properties, and the in vitro tests.