Study on the effects of flg22 in Arabidopsis stomatal development

• Main Authors: Tzu-Hsuan Kao, 高子萱
• Other Authors: Chien-Chih Yang
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/997mg8

碩士 === 國立臺灣大學 === 生化科技學系 === 106 === Flg22, a 22-amino-acid peptide derived from flagellin, is known to activate the pattern triggered immunity (PTI) in Arabidopsis. With the short-term treatment of flg22 for 1 hr, the defense system of Arabidopsis is triggered including stomatal closure to prevent pathogen invasion. The effects of long-term treatment of flg22 for 7 to 14 days in Arabidopsis are root growth inhibition, callose deposition and expression of pathogenesis related genes. The effect of flg22 in regulating plant development was investigated in this study. Stomatal development is one of the indicators in response to environment change. Arabidopsis was treated with flg22 for 3 days to observe if flg22 affected stomatal development in this study. In this research, the bioactivity of synthetic flg22 was demonstrated by inhibiting the root growth of wild type (WT). Some of the stomata in WT treated with flg22 showed irregular patterning which “one-cell spacing rule” was not strictly followed. The stomatal index decreased in a dose-dependent manner in WT treated with flg22. The expression levels of those master regulator genes and their interacting partners in stomatal development was changed in WT treated with flg22. In this study, flg22 was proposed to play roles in stomatal development by regulating the transcript levels of master regulators that leads to the decreased stomatal density and impaired stomatal patterning. Keywords: Arabidopsis; stomatal development; peptide; flg22