| Summary: | 碩士 === 國立臺灣大學 === 醫學檢驗暨生物技術學研究所 === 106 === Staphylococcus aureus is one of the human normal inhabitant resident microbiota. It is an important human pathogen responsible for many infectious diseases including skin and soft tissue infections (SSTIs), pneumonia, endocarditis and blood-stream infections. S. aureus will be classified as healthcare-associated or community-associated. Prevalence of methicillin-resistant S. aureus (MRSA) and its ability to resist multiple drugs has posed a serious challenge. S. aureus is one of the common infections from skin and soft tissue. Because most of the staphylococcal SSTIs has recurrence, patients do not have good immune response to the infection. Therefore, the aim of our study is to know the characterization of S. aureus isolated from skin and soft tissue infection.
We collected 129 isolates from emergency and/or outpatient and 91 isolates from hospitalized patients. Among all isolates, 172 of them are from skin and soft tissue infection. MRSA from emergency and outpatient is more sensitive than hospitalized one, but no significant difference in MSSA. In the distribution of spa types, 3% of SSTI isolates are t091 and t338, 4% is t189, 8% is t008, 12% is t037 and 16% is t437. Among MRSA, 54% of isolates from emergency and outpatient and 24% of isolates from hospitalized patient is pvl- positive. Among MSSA, 17% of isolates from emergency and outpatient and none of isolates from hospitalized patient is pvl- positive.
Despite of erythromycin and trimethoprim/sulfamethoxazole, pvl- positive MRSA is less resistant. Most of the SSTI isolates in our study are IEC type B. Most of the SSTI isolates in our study are hla- positive and hld- positive. The most frequent enterotoxin is seb in classical Staphylococcal enterotoxins and seh in other enterotoxins. Five SSTI isolates in our study are tst- positive. 4 of the 9 bbp- positive isolates are t091. We have found one isolate with edin no isolate with lukM and most of the isolates with lukE-lukD.
In this study we examined the characterization of S. aureus isolate from skin and soft tissue infection preliminary. Most of the results in our study are similar with others although there is a little difference. The geographical differences and the sample origin may cause the divergence and need more experiment to explore.
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