| Summary: | 碩士 === 國立臺灣大學 === 生醫電子與資訊學研究所 === 106 === Bacteria identification and characterization reveal much important information in various fields, such as: food safety, water quality, sepsis and so on. Surface-enhanced Raman scattering (SERS) has been applied for bacteria identification due to its highly specific and non-invasive features. However, the SERS spectra of bacteria are usually very complicated due to their complex metabolite composition of purine derivatives. To address the above problem, we developed a microfluidic platform which enables on-chip liquid chromatography (LC) separation of multiple molecules followed by in situ SERS detection. A mixture of fluorescent FITC and R6G dyes was tested in the system to confirm its functionality. Their retention times were revealed by time-lapsed fluorescence imaging and SERS detection. Next, the system separated a mixture of hypoxanthine and adenine— the main purine metabolites of E. coli—and identified them with acquired SERS signatures. Then, a uracil and adenine mixture was tested to evaluate the performance of the platform. In the end, an ex situ SERS detection was introduced. The LC-SERS microfluidic platform holds two important features. First, the developed microfluidic LC column is just few centimeters long that is one order of magnitude shorter than commercial HPLC columns. Second, the microfluidic flow control system connected to the LC-SERS microfluidic device allows for automatic and real-time fluidic control, greatly reducing sample consumption and preventing any potential cross contamination. Accordingly, this highly integrated and miniaturized LC-SERS microfluidic system could greatly facilitate identification and monitoring of bacteria, relocating the entire process from modern microbiology laboratories with expensive facilities to actual clinical settings, which in turn enables punctually available diagnosis data.
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