Assessing Effect of the Transgenic SlMYB94 Allele on Floral Organ Length in Tomato

• Main Authors: Pin-Yao Chen, 陳品堯
• Other Authors: Kai-Yi Chen
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/ngdab4

碩士 === 國立臺灣大學 === 農藝學研究所 === 106 === The tomato se2.1 is a composite quantitative trait locus, including style2.1, stamen2.1, stamen2.2, stamen2.3, and dehiscence2.1. The stamen2.2 was delimited between molecular markers cLED19A24 and CT9 on the chromosome 2. In the current study, the SlMYB94 gene is one of genes residing in the aforementioned chromosomal region and was chosen as the candidate gene of stamen2.2. The correlation between the SlMYB94 copy number and the changes of stamen and style length were observed via the gene transformation approach. CaMV 35S marker and genetic background marker were used to identify successful transgenic plants in the T_0 generation. In the T_1 generation, pyrosequencing was used to detect the proportion of single nucleotide polymorphism between the transformed allele and the background allele, and hence to infer genotypes of T_1 plants. The results showed that the “sinica2-9” T_0 plant successfully received a single copy of the SlMYB94 transgenic allele but its T_1 progenies, the 16TS357 population, did not show significant difference on style length and stamen length between individuals carrying different copy number of the SlMYB94 transgenic alleles. This conclusion infers that the SlMYB94 gene is not stamen2.2.