Study of the hemostatic performance of chitosan-based wound dressings via a flow-through device

• Main Authors: Yen-Cheng Chen, 陳晏誠
• Other Authors: 李雨
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/ejze68

碩士 === 國立臺灣大學 === 應用力學研究所 === 106 === Besides many static tests, there are two types of dynamic devices and methods for testing the hemostatic performance of wound dressings (or gauzes) in a flowing environment, and are called here the constant pressure head (CPH) device and the constant flow rate (CFR) devices, respectively. The “mass through the device” and the “pressure drop across the gauze section” were measured, respectively, for the CPH and CFR devices, for accessing the hemostatic performance when test fluids (human whole blood here) were let to flow through the gauzes. Both devices have their advantages and disadvantages. The goal of the present study is to develop a dynamic flow-through device which possesses the characteristics of both the CPH and CPR devices. There are one inlet and two outlets in the present proposed device. The test blood was injected at a constant flow rate into the device, and then divided into two streams: the main stream without gauzes in its passage, and the secondary stream flowing through the test gauzes. Both the time evolution of the pressure drops across a section of the main stream channel and that of the secondary stream channel (containing the gauze section) and the flow-through blood masses at both outlets were measured for accessing the dynamic hemostatic performance of gauzes. The gauze possesses a better hemostatic performance when the associated pressure drop is higher and the flow-through blood mass is less in the secondary channel. The device has three parts which are made of PDMS, and are bonded together. The parts can be separated and re-bonded easily for exchanging the gauze samples. The aggregation of red blood cells on gauzes after test was examined for understanding some of the details of hemostasis. The blood flow in the present device is more closely related to the bleeding at wound in comparison with those in the CPH and CFR devices. Some findings from the present experimental study are as follows. (1) It has been proved that the present device is an effective and easy-operated device, for accessing the hemostatic performance of vary gauzes. (2) For the chitosan-based gauzes, the acidification of gauzes can enhance substantially the hemostatic performance, and is more effectively by comparing with the enhancement associated with increasing the chitosan content in the gauzes. It is hoped that the present study is helpful for the development of more effective gauzes for hemostasis, for better understanding of the hemostatic mechanisms, and for the new researchers who wish to devote to the related subject.