Development of microtissue based on chitosan hydrogel

• Main Authors: Kui-Yu Pan, 潘奎宇
• Other Authors: Yu-Hsiang Hsu
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/3y2tbw

碩士 === 國立臺灣大學 === 應用力學研究所 === 106 === The aim of the 3-D bio-printing is to build functional 3-D biomimetic tissues in vitro. However, the current challenge is o build biomimetic tissues that can provide functional vasculature to supply oxygen, nutrition and metabolism similar to microvascular network in vivo. In tissue engineering, it is important that 3-D gel contains appropriate matrices, and 3-D cell scaffold has functional vessel network in vitro. Currently, the method of building microvascular network is to use hydrogel composed with extracellular matrix (ECM) embedded with endothelial cells and stromal cells. However, common ECM-based hydrogel like fibrin gel, collagen gel, and matrigel are not suitable for 3-D printing process. On the other hand, not all of 3-D printable gels have the potential for induction of angiogenic and vasculogenic process. In this thesis, the 3-D printable chitosan gel was used as the base of the 3-D printable hydrogel, and different extracellular matrix is added to study their contribution to the development of vasculature. The extracellular matrix been studied includes Fibrinogen and collagen-I. It is found that vasculogenic process is hard to be induced in a mixture of chitosan and fibrin gel. But, the level of vessel formation is increased with mixtures composed of both fibrinogen and collagen-I fibers. Using a bioreactor developed in this study to create a hypoxic environment for stimulating vasculogenesis, it is found that the addition sequence of chitosan, fibrinogen, and collagen-I can influence the development of vasculogenic process. After comparing the developed vessel structures on Day-5, it is found that a better vessel formation can be created by adding fibrinogen between chitosan and collagen-I. Discussion on the experimental findings and statistical analysis on the developed vasculature are detailed in this thesis.