| Summary: | 碩士 === 國立臺灣大學 === 臨床牙醫學研究所 === 106 === Rheumatoid arthritis is a systemic disease that is regulated by a variety of cells, including monocytes, synovial cells, endothelial cells, and other immune cells. Rheumatoid arthritis is also a risk factor for cardiovascular disease. A similar cellular composition to atheroma was found in the synovial fluid of the patient. 27-hydroxycholesterol exacerbates atherosclerosis. In arthritis, rheumatoid arthritis synovial cells (RASF) play an important role. This experiment hopes to investigate the regulation of sterol 27-hydroxylase (CYP27A1) stimulated by fatty acid in HepG2 (human hepatocarcinoma cell line), and whether RASF will be affected by 27-hydroxycholesterol to explore obesity and cholesterol metabolism for rheumatoid arthritis. Impact. The experimental results showed that the protein expression of CYP27A1 was increased in human hepatoma cell lines stimulated by oleic acid (oleic acid). And the 27HC content in the culture solution increased. In another experiment, 27HC promoted the proliferation of RASF cells; and caused an increase in the concentration of various inflammatory mediators. And in animal experiments, it is found that the diet of obesity can indeed aggravate the development of arthritis, the infiltration of inflammatory cells and the proliferation of synovial cells, while the mice used in the anastrozole that inhibit CYP27A1 have symptoms of fatty liver and arthritis. Reduced. Summarizing the above findings, obesity will change the lipid metabolism of liver cells and increase CYP27A1, and produce a larger amount of 27HC, which may be the cause of arthritis.
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