Development of Wavelength Calibration Procedure for a Self-developed Chip-spectrometer and its Application in Urine Protein Test Strip Measurement

• Main Authors: Wei-Huai Chiu, 邱韋懷
• Other Authors: Cheng-Hao Ko
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/29cmnf

碩士 === 國立臺灣科技大學 === 自動化及控制研究所 === 106 === A conventional spectrometer consists of a sophisticated arrangement of optical components and is difficult to assemble.The micro-spectrometer can perform spectrum measurement more precisely through calibration. And use the protein liquid to establish a reflectivity versus concentration curve that unknown concentration of protein is determined by the change of absorption peak at a specific wavelength. In our research use multi-channel fiber coupled laser source(406 nm、520 nm、635 nm、642 nm、670 nm、785 nm、850 nm、980 nm) to verify spectrum resolution and complete wavelength calibration in real-time image. After the Rayleigh criterion to verify average spectral resolution better than 3 ~ 5nm. The characteristics of specific wavelength laser are highly vertical-aligned and narrow beam spectrum that applied to calibrate wavelength position. The purpose of wavelength calibration is to convert the pixel values measured by the image sensor receiver of the spectrometer to wavelengths values. After the above calibration is completed that we can start to measure different concentrations (0 mg/dl、15 mg/dl、30 mg/dl、100 mg/dl、300 mg/dl、1000 mg/dl) of protein liquid then observed the reaction of spectrum on urine strips. The relationship of the spectrum in the detection area and the standard white area, called reflectivity. Where obvious changes occur are called absorption peak at 623.75nm.We can establish a reflectivity versus concentration table called R-C table, calculate the unknown concentration by micro-spectrometer. Finally, we compare performance with Ocean optics HR4000.