Study on the optimum condition of mycelial growth and fruiting body production of Cordyceps cicadae

• Main Authors: WANG, YU-TING, 王郁婷
• Other Authors: LO, CHAUR-TSUEN
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/7mg373

碩士 === 國立虎尾科技大學 === 生物科技系碩士班 === 107 === [Cordyceps cicadae (Miq.) Massee] is an sexual stage of Isaris cicadae Miq. [Syn. Paecilomyces cicadae (Mique) Samson] has been used as a valuable Chinese medicine, which was known with its immunomodulatory, antipyretic, anti-fatigue ability. The artificial cultivation of C. cicadae is the best way to solve the difficulty of obtaining natural C. cicadae, the high price, the less production area and the long interval of harvesting fruiting bodies (FB). My thesis detected that using sucrose as carbon source and cicada shell meal as nitrogen source at 24 °C, pH 5.5, 24 hours light resulted the longest of the C. cicadae mycelium. Solid culture 1 % maltose+ 5 % fish meal condition increased 58.5% average of fruiting bodies (FB) height (4.58 cm) as compare to 1 % glucose+ 5 % fish meal (2.89 cm). Moreover, the optimal water content for culture FB is 50%. Incubated C. cicadae for 3 days had the suitable biomass of mycelia and spore density (1×107 spores/ml) for pre-activated spore for farther rice-solid culture. In addition, it will be discussed whether C. cicadae FB can be dyed in the medium by adding the natural pigment.