Effects of the Brazilian Mushroom Extract and It’s Polysaccharides on Wound Healing After Radiation Injury

• Main Authors: Guan, Jen-Sheng, 官振聖
• Other Authors: LIU, WEI-CHUNG
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/a727d3

碩士 === 慈濟科技大學 === 放射醫學科學研究所 === 106 === Abstract Brazilian mushrooms are well-known traditional medicinal mushrooms, and studies have shown that they have certain effects on cellular immunity, anticancer activity, and radiation protection. The purpose of this study is to assess the potential and possible mechanism of Brazilian mushroom extract (BM) and polysaccharide (BMP) on wound healing after radiation injury. In this study, mouse fibroblasts (NIH-3T3) were used to model the model of cancer patients with local irradiation and wound healing after surgery. In wound healing, many factors may lead to impaired wound healing that slows down the rate of repair, delays healing, and even does not heal. The experimental conditions were divided into six groups, including the control group, Brazilian mushroom extract group, Brazil mushroom polysaccharide group, radiation control group (5 Gy), radiation plus Brazil mushroom extract group, and radiation plus Brazilian mushroom polysaccharide group. Brazilian mushroom polysaccharides are high-molecular polysaccharides, which can induce many physiological reactions and regulate physiological functions. The results also show that the Brazilian mushroom extract and polysaccharides have the ability to promote wound healing in cells, confirming accelerated healing by ERK and NF-κB. The polysaccharides inhibit the production of ROS after irradiated by cells. At the same time, the polysaccharides also prevented the G2/M phase arrest of fibroblasts in NIH-3T3 mice after radiation injury. Extracts and polysaccharides both reduced apoptosis and increased cell migration rate after radiation injury. From the results, The Brazilian mushroom extract and polysaccharide stimulated ERK and NF-κB secretion in fibroblasts of NIH-3T3 mice to induce cell activation to increase the rate of inflammation, proliferation, and migration of cells to accelerate wound healing. Presumably related to its Ras and MMP-9 pathway.