Visible Light Induce the Bio-Inactivation with the Biocompatible Materials

• Main Authors: MING-YEH YANG, 楊明曄
• Other Authors: Anren Hu
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/9bx85k

博士 === 慈濟大學 === 醫學科學研究所 === 106 === Based on the photochemical properties of photodynamic therapy, the light-induced inactivation of pathogenic microbial was investigated. The photosensitizer with light can trigger or catalyze the degradation of the chemical molecules and generation of reactive oxygen species (ROS). The UV and blue light could make microbial inactivation, but the short wavelength lights with higher energy may damage cells. By appropriate photosensitizer, the irradiation of visible blue lights with longer wavelength, that also produce the inactivation of pathogenic microbial under the specific conditions. The photodynamic therapy with blue light and photosensitizer will be a simple and safe technique on biomedical fields. The wavelength of emission maximum of blue light was 462 nm that used in this study. The organic and inorganic material was selected as evaluation for the photosensitive and antimicrobial ability. (1) The visible light irradiation could induce the antimicrobial potential of ZnO nanoparticles on drug-resistant pathogen: Acinetobacter baumannii is a common multidrug-resistant pathogen in hospital and induce the nosocomial infection. The ZnO-NP-photo system, ZnO nanoparticles at a concentration of 0.125 mg/mL combined with 462 nm wavelength of blue light at an intensity of 10.8 J/cm2, could destroy the cytomembrane without DNA damage. This system could also inhibit the Propionibacterium acnes (P. acnes), Candida albicans and Colletotrichum gloeosporioides. (2) The visible light irradiation could induce the antimicrobial potential of curcumin on P. acnes: The P. acnes have opportunistic infection in human skin and cause acne vulgaris. The curcumin-photo system, curcumin at 1.52 µm combined with 462 nm wavelength of blue light at an intensity of 0.09 J/cm2, could damage the P. acnes through the cytomembrane. Otherwise, the curcumin could reduce to vanillin through the visible light irradiation. (3) The visible light irradiation could induce the anticancer potential of riboflavin on HeLa cancer cell: The riboflavin has blue light sensitive and could release the ROS during irradiation process. This system could enhance the p53 and Caspase 9 protein level in HeLa cell and toward the apoptosis. This study evaluated the differential biocompatibility objectives from organic and in-organic material that antimicrobial and anticancer ability under visible light irradiation.