Differences of Lignocellulose of Mushroom Bags Saccharification by Acid-Base Hydrolysis

• Main Authors: LEE, CHENG-RONG, 李承融
• Other Authors: LIN, CHUN-PING
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/p59gbv

碩士 === 亞洲大學 === 食品營養與保健生技學系 === 106 === The aim of this project was to develop bioethanol from lignocellulose saccharification, which solves the problem of waste mushroom logs of 470 million packages that are discarded as rubbish every year in Taiwan. It is advantageous for dealing with the petroleum crisis and the agricultural waste problem, to find an appropriate alternative energy or green energy in currently Taiwan trend. The waste mushroom bags have been found to contain a large amount of lignocellulose that exceeds more than 75 mass % for per kilogram, but it is difficult to convert into sugar from the lignocellulose of waste mushroom bags, which contain many impurities. Thus, the impurities interfere with saccharification reaction. Therefore, the methods of chemical and physical for hydrolyzing of lignocellulose for converting to sugar were compared, with the goal of finding the suitable methods for the saccharification reaction of the lignocellulose in this study. Meanwhile, this study was conducted the containment of waste mushroom bags’ composition analysis, and then to acquire the crude fiber content of 57.32 mass%. The results of lignocellulose analysis contain the cellulose, hemicellulos, and lignin were 15.04, 14.19, and 77.78 mass%, respectively. In addition, the lignocellulose was pretreated by acid-base hydrolysis under the high temperature of water-bath and the high pressure. Therefore, the content of total sugar (75.05 mg/g) was measured by hydrolysis with 1 N H2SO4 for 3 times, which was obtained the highest rate is 12.79 mass%.