Determination of Hepatotoxic Pyrrolizidine Alkaloids in Tussilage farara L. Products and Tournefortia sarmentosa Lam.

• Main Authors: WEI, PO-CHENG, 衛柏成
• Other Authors: YANG, YA-CHEN
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/kmecq8

碩士 === 亞洲大學 === 食品營養與保健生技學系 === 106 === Alkaloid extracts were analysed to detect and quantify the pyrrolizidine alkaloids in Tussilage farara L. and Tournefortia sarmentosa Lam. using gas-chromatography mass spectrometry (GC/MS), and further utilize HPLC and NMR to purified and identified the major PAs 9-angeloylretronecine of T. sarmentosa. In T. farara part, the samples include dry plants, scientific Chinese medicine and Chinese medicine are collected from pharmacy stores. We have investigated eleven PAs: 7-angeloylretronecine, 9-angeloylretronecine, 7-tigloylplatynecine, 9-angelylplaty- necine, sencecionine, bulgarsenine, integerrimine, platyphylline, neoplatyphylline, senkirkine, neosenkirkine. Among them, seven PAs are detected for the first time. The main cause of the toxicity PAs as otonecine type of macrocyclic diester of senkirkine, although it’s toxicity in this 11 PAs are not the strongest, it’s content than the rest of PAs extra times. The total content of PAs in scientific Chinese medicine was 24.93±9.04~55.54±14.21 μg/g, while that of Chinese medicine was 0.24~8.72 μg/g. In the T. sarmentosa part, all samples purchased from traditional Chinese medicine stores from Taiwan. After analysis by GC/MS, We have investigated 17 PAs: 9-angeloyltrachelamthamidine, 9-senecioyltrachelamthamidine, 9-tigloyltrachelam- thamidine, unknown, 7-angeloylretronecine, 9-angeloylretronecine, macrophylline, 7-senecioylretronecine/7-tigloylretronecine, 9-senecioyletronecine, macrophylline isomer, 9-tigloylretronecine, viridiflorine, supinine, amabiline/coromandalinine, lycopsamine, rinderine, 3'-acetylechinatine/3'-acetyllycopsamine, which only supinine been reported in previous report, and the remaining PAs are detected for the first time. These 17 PAs are all monoester. The major PAs is 9-angeloylretronecine, a retronecine type, it’s toxicity is weaker than senkirkine. The average total PA concentration varied from 25±3.8~69.51±2.57 μg/g in T. sarmentosa. The quantitative results are based on recommended dietary allowance by EFSA and taken according to appropriate method, there will be no possibility of poisoning in the short term, but it’s still necessary to pay attention to the hepatotoxicity and tumorigenesis in the long term. This study will provide the qualitative and quantitative as an assessment of the use of Chinese medicines, thereby reducing the toxicity caused by the treatment.