| Summary: | 碩士 === 國立臺北科技大學 === 化學工程與生物科技系生化與生醫工程碩士班 === 106 === Previous laboratory results, the down expression of microRNA-378(miR-378) was observed in high-grade colon cancer. Besides, upregulated miR-378 expression could inhibit the cell proliferation. Many reports showed that miR-378 is associated with cell proliferation. In 2012, the Europe’s study reported miR-378 had low expression, but the China’s study reported miR-378 had high expression in bladder cancer. We therefore aim to investigate the role of miR-378 in bladder cancer. In this study, we chose three bladder cancer cell lines (MGH-U1, MGH-U3, MGH-U4) and one normal bladder epithelial cell immortalized cell line (sv-HUC1) which was used as control groups. Each cell lines were performed using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay to quantification. The low expression miR-378 cell lines were transfected miR-378, while the high expression cell lines were inhibited, 48 hours. Then, MTS assay was used to detect cell proliferation, and the expression of IGF1R, AKT, p-AKT protein were observed by Western blotting, and the cell cycle was measured by flow cytometry. The results showed that only MGH-U3 was shown high expression of miRNA-378 when compare to the control group, while the high grade cell line MGH-U1 showed the lowest expression of miR-378. Those bladder cancer cell lines which doubling time, and grade level both were negative correlation with the miR-378 expression. The low level cell lines which cell proliferation and cell cycle were decrease, IGF1R, AKT, p-AKT were down-regulate. And high level cell lines were up-regulate, after transfection or inhibition miR-378. These results showed that miR-378 expression level high and low might be correlated with grade level of bladder cancer cell lines. Hopefully, the finding may be used as an indicator of bladder cancer malignancy in the future.
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