Ethanol Production from Cellulase Containing Sugarcane Bagasse Produced by Solid State Fermentation Method

• Main Authors: Cheng Cheng, 鄭誠
• Other Authors: Kow-Jen Duan
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/k44z7d

碩士 === 大同大學 === 生物工程學系(所) === 106 === Sugarcane bagasse was pre-treated by 1% H2SO4 followed by 2% NaOH. Each pre-treatment process was carried out at 120C for 40 min. The pre-treated sugarcane bagasse (SCB) supplemented with wheat bran in a weight ratio of 4:1 was used as the substrate for cellulase production by solid state fermentation using Trichoderma reesei RUT C30 at 30 C. Cellulase activity of 13.70 2.81 FPU / g-DS was achieved after 6 days’ incubation. 5% (w/v) cellulase containing SCB (CSCB) was directed hydrolyzed at 50C for 4 h, then the hydrolyzed CSCB slurry was cooled to 40C, thermotolerant yeast (Kluyveromyces marxianus) was pitched to the slurry for ethanol production in simultaneous saccharification and fermentation (SSF) process. The yield of ethanol was 14.10.8% (w/w) from CSCB. Ethanol yield of 30.30.4％（w / w）and 27.50.1% (w/w) was achieved from CSCB supplemented with with 5 FPU/g-DS CTec 2 or 10 FPU/g-DS cellulase produced from the Institute of Nuclear Energy Research. The ethanol yield of 36.80.4% from glucose or 30.10.5% from deactivated CSCB plus 15 FPU/g-DS CTec2 was achieved respectively from our ethanol fermentation system. This study proved that direct employ CSCB to produce ethanol can reduced the cost of commercial cellulase.