The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor
碩士 === 國立雲林科技大學 === 電子工程系 === 106 === In this research, development of polydopamine modified AuNPs/AuNPs/TiO2 nano-composite films for LSPR glucose sensors are presented. Gold nanoparticles (AuNPs) are fabricated by using photochemical method, and then two different sizes of AuNPs are sequentially d...
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ndltd-TW-106YUNT03930542019-05-16T00:44:55Z http://ndltd.ncl.edu.tw/handle/488vrw The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor 以聚多巴胺修飾金奈米粒子-二氧化鈦奈米複合薄膜 應用於侷部表面電漿共振葡萄糖生物感測器之研究 HUANG, WEI-HAO 黃偉豪 碩士 國立雲林科技大學 電子工程系 106 In this research, development of polydopamine modified AuNPs/AuNPs/TiO2 nano-composite films for LSPR glucose sensors are presented. Gold nanoparticles (AuNPs) are fabricated by using photochemical method, and then two different sizes of AuNPs are sequentially deposited on TiO2 film using APTES-assembly method and TBBT-layer-by-layer method, respectively. A LSPR sensors optimized by using polydopamine deposited on AuNPs/AuNPs/TiO2 nano-composite films are used for glucose biosensors, and the performance of biosensors are investigated. In order to determine the optimal condition of nano-composite films for detecting different concentrations of glucose solutions, the environmental sensing of different refractive indices using various nano-composite films are investigated. The AuNPs30nm/AuNPs10nm/TiO2 nano-composite films and PDA/AuNPs30nm/AuNPs10nm/TiO2 nano-composite films show the refractive index sensitivity (RIS) of 212.5 nm/RIU and 162.5 nm/RIU, respectively, and the linearity of these two nano-composite films are R2 = 0.929 and R2 = 0.990, respectively. After depositing PDA on the AuNPs30nm/AuNPs10nm/TiO2 nano-composite films, the sensitivity is decreased but the linearity is increased, and these results indicate the sensing stability increased by depositing the PDA layer, on the other hand, it is also the main cause of the decrement in the sensitivity. Finally, the performance of the GOx/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films and the GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films are compared, the results indicate that the GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films have better performance with a sensitivity of 0.495 nm/RIU, a linearity of R2 = 0.987, and a limit of detection of 5.4 mg/dL (0.3 mM). The GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films also exhibited great specificity, reproducibility, and a lifetime of 3 days in room temperature (25 °C). These results indicate that GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films make it promising for the fabrication of glucose biosensors. CHOU, HSUEH-TAO 周學韜 2018 學位論文 ; thesis 198 en_US |
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碩士 === 國立雲林科技大學 === 電子工程系 === 106 === In this research, development of polydopamine modified AuNPs/AuNPs/TiO2 nano-composite films for LSPR glucose sensors are presented. Gold nanoparticles (AuNPs) are fabricated by using photochemical method, and then two different sizes of AuNPs are sequentially deposited on TiO2 film using APTES-assembly method and TBBT-layer-by-layer method, respectively. A LSPR sensors optimized by using polydopamine deposited on AuNPs/AuNPs/TiO2 nano-composite films are used for glucose biosensors, and the performance of biosensors are investigated.
In order to determine the optimal condition of nano-composite films for detecting different concentrations of glucose solutions, the environmental sensing of different refractive indices using various nano-composite films are investigated. The AuNPs30nm/AuNPs10nm/TiO2 nano-composite films and PDA/AuNPs30nm/AuNPs10nm/TiO2 nano-composite films show the refractive index sensitivity (RIS) of 212.5 nm/RIU and 162.5 nm/RIU, respectively, and the linearity of these two nano-composite films are R2 = 0.929 and R2 = 0.990, respectively. After depositing PDA on the AuNPs30nm/AuNPs10nm/TiO2 nano-composite films, the sensitivity is decreased but the linearity is increased, and these results indicate the sensing stability increased by depositing the PDA layer, on the other hand, it is also the main cause of the decrement in the sensitivity.
Finally, the performance of the GOx/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films and the GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films are compared, the results indicate that the GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films have better performance with a sensitivity of 0.495 nm/RIU, a linearity of R2 = 0.987, and a limit of detection of 5.4 mg/dL (0.3 mM). The GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films also exhibited great specificity, reproducibility, and a lifetime of 3 days in room temperature (25 °C). These results indicate that GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films make it promising for the fabrication of glucose biosensors.
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author2 |
CHOU, HSUEH-TAO |
author_facet |
CHOU, HSUEH-TAO HUANG, WEI-HAO 黃偉豪 |
author |
HUANG, WEI-HAO 黃偉豪 |
spellingShingle |
HUANG, WEI-HAO 黃偉豪 The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor |
author_sort |
HUANG, WEI-HAO |
title |
The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor |
title_short |
The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor |
title_full |
The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor |
title_fullStr |
The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor |
title_full_unstemmed |
The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor |
title_sort |
research of polydopamine modified aunps-tio2 nanocomposite films applied for localized surface plasmon resonance glucose biosensor |
publishDate |
2018 |
url |
http://ndltd.ncl.edu.tw/handle/488vrw |
work_keys_str_mv |
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