The Research of Polydopamine modified AuNPs-TiO2 Nanocomposite Films Applied for Localized Surface Plasmon Resonance Glucose Biosensor

• Main Authors: HUANG, WEI-HAO, 黃偉豪
• Other Authors: CHOU, HSUEH-TAO
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/488vrw

碩士 === 國立雲林科技大學 === 電子工程系 === 106 === In this research, development of polydopamine modified AuNPs/AuNPs/TiO2 nano-composite films for LSPR glucose sensors are presented. Gold nanoparticles (AuNPs) are fabricated by using photochemical method, and then two different sizes of AuNPs are sequentially deposited on TiO2 film using APTES-assembly method and TBBT-layer-by-layer method, respectively. A LSPR sensors optimized by using polydopamine deposited on AuNPs/AuNPs/TiO2 nano-composite films are used for glucose biosensors, and the performance of biosensors are investigated. In order to determine the optimal condition of nano-composite films for detecting different concentrations of glucose solutions, the environmental sensing of different refractive indices using various nano-composite films are investigated. The AuNPs30nm/AuNPs10nm/TiO2 nano-composite films and PDA/AuNPs30nm/AuNPs10nm/TiO2 nano-composite films show the refractive index sensitivity (RIS) of 212.5 nm/RIU and 162.5 nm/RIU, respectively, and the linearity of these two nano-composite films are R2 = 0.929 and R2 = 0.990, respectively. After depositing PDA on the AuNPs30nm/AuNPs10nm/TiO2 nano-composite films, the sensitivity is decreased but the linearity is increased, and these results indicate the sensing stability increased by depositing the PDA layer, on the other hand, it is also the main cause of the decrement in the sensitivity. Finally, the performance of the GOx/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films and the GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films are compared, the results indicate that the GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films have better performance with a sensitivity of 0.495 nm/RIU, a linearity of R2 = 0.987, and a limit of detection of 5.4 mg/dL (0.3 mM). The GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films also exhibited great specificity, reproducibility, and a lifetime of 3 days in room temperature (25 °C). These results indicate that GOx/PDA/AuNPs30nm/AuNPs10nm/TiO2 glucose sensing films make it promising for the fabrication of glucose biosensors.